This new novel SNP FGC5496 was found in 0FW1RA (MJost), a Full Genome Y-Chrome tested DF13* person and matched to person with a public dataset: PGP50 (CG, R-DF13), along with FGC5495. YSeq.net has utilized Sanger Sequencing and resulted in confirming that FGC5496 has a clearly G+ mutation. In testing a second 0FW1RA novel SNP found in PGP50, FGC5495, has a high similarity to ChrX and resulted in a Sanger test failure as suspected.

There were 60 plus new YSNPs non-novel discovered in the FGC-0FW1RA and they were compared to other public datasets Haplogroups in the neighborhood of R-DF13 were considered; included 42 datasets covering Haplogroups: DF49/S474, DF1/L513/S215, L144.1/S175, Z255/S219, Z253/S218, DF21/S192, DF41/S524/CTS2501, Z251 & CTS4466 and were found that they were either had a reference allele as mostly ancestral ("-"); or infrequently as "x"=ambiguous or "?"=no-read/no-calls. There were no samples that had an allele that was derived ("+").

The FGC5496 marker should be of interested for R1b-DF13 researchers and is certainly the SNP that would be prefer for phylogenetic purposes as per Thomas Krahn.

With the above information and results, it appears that this new DF13 novel subclade, designated as FGC5496, is an very old Haplogroup under DF13.

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